GERBERA MICROPROPAGATION PDF

PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .

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In the present study, experiments were conducted to investigate organogenesis from various sources of Gerbera explants.

Ggerbera technology is being utilized commercially in the ornamentals industry and in other plant production organizations worldwide Chu, ; Huetteman and Preece, ; Mantell et al. On Tu-er-fang derived from Gerbera plants. Relatively, in this experiment, callus formation was optimum when leaf explant was cultured on MS medium supplemented with gerbfra. A potent cytokinin for woody plant tissue culture. Propagation Ornamental Plants, 4: High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L.

Micropropagation of Gerbera (Gerbera jamesonii Bolus).

Callus is capable of forming adventitious roots. Organogenesis and histogenesis of adventitious organ induced on leaf blade segments of Begonia elatior hybrids Begonia x hiemalis in tissue culture. Studies of Gerbera clonal propagation could also be efficiently adapted for other ornamental plants.

Growth Optimization and Organogenesis of Gerbera mlcropropagation Bolus ex. Gerbera plantlets formed were transferred to soil with Sterilized seeds were cultured on basal MS Murashige and Skoog, medium. The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support.

Plant regeneration via direct organogenesis is much preferred over regeneration through somatic embryogenesis and callus culture Arockiasamy et al. BAP strongly enhanced regeneration of shoots in petiole explants of Gerbera jamesonii.

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In vitro propagated Gerbera plants were compared morphologically with the intact plant.

Addition of auxins together with cytokinins becomes essential for shoot induction and multiplication depending on the plant type. Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year. However, regeneration of root was not observed when explants were cultured on MS medium supplemented with BAP and 2, 4-D.

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Petiole explants have been identified as the most regenerative explants for multiple shoot formation. Root explants itself also induced the formation of root in vitro. In vitro response of plant tissues depends on genotype, the physiological status of the donor plant, the type of explant, the culture medium and their interactions Tosca et al. Micropropagation of Ornamental Plants.

Slow-growing endogenous bacteria are one of the most important problems in gerbera micropropagation but require more studies on control and prevention. Ornamental plants and woody plant species are also propagated commercially by axillary bud proliferation Mantell et al.

How to cite this article: In vitro regeneration of Hagenia abyssinica Bruce J. Petioles and leaves obtained from aseptic young plantlets formed from the seedlings were used as source of explants. In Begoniathe addition of kinetin and zeatin in the culture medium Murashige and Skoog, induced multiple shoots Jain, However, in Gerberaaddition of charcoal is not necessary for rooting.

Numerous types of explants and protocols for micropropagation have been established and used for gerbera. Kluwer Academic Publishers, Dordrecht, pp: Tissues of explants generally show distinct planes of cell division, various specializations of cells and organization into specialized structures such as the vascular system.

Takayama and Misawa reported that medium containing 0. Gerbera jamesonii Bolus ex. Plant hormones and type of explants play very important roles in determining regeneration of Gerbera jamesonii in vitro.

Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al.

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Many commercial ornamental plants are being propagated by in vitro culture on the culture medium containing auxins and cytokinins Preil, ; Rout and Jain, This species consists of many cultivars with variety of colors and shapes and they are popular commercial plants. Highest numbers of shoots from petiole explants 9. One of the most important techniques in plant improvements is via micro propagation. Optimilization of Gerbera plantlet production from excised capitulum explants.

Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture. Thus, improvements of plant quality need to be studied and more research needs geebera be mifropropagation. The combination of low concentration of cytokinin and auxin initiated propagation of Begonia species Reuter and Bhandari, Based on the present study, all combinations of hormones used were able to induce callus.

In conclusion, the research done has proven that micro propagation of Gerbera jamesonii Bolus ex. Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture supplemented with 2.

MICROPROPAGATION OF GERBERA | ICAR-Indian Institute of Horticultural Research

Pakistan Journal of Biological Sciences Volume 11 The main purpose for propagation of ornamental plants is for its aesthetic value. Shoot formation was observed when capitulum explants were cultured on MS modified medium supplemented with 2. Shoot tips are the commonly used explant while adventitious shoot induction from the capitulum is also a popular method. However, in this study, at a very high concentration of BAP and other growth regulators, development and growth of shoots were inhibited.

This research was funded by Vote F Grant No. Structural, physiological and biochemical aspects.

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